白癜风治疗医院 http://pf.39.net/bdfyy/bjzkbdfyy/ClonalHematopoiesis-DriverDNMT3AMutationsAlterImmuneCellsinHeartFailureCirculationResearchresearch-articleEarlyRecent,Nov06,./CIRCRESAHA..本文由“天纳”临床学术信息人工智能系统自动翻译点击文末“阅读原文”下载本文PDFRationale:Clonalhematopoiesis(CH)drivenbymutationsofDNAmethyltransferase3a(DNMT3A)isassociatedwithincreasedincidenceofcardiovasculardiseaseandpoorprognosisofpatientswithchronicheartfailure(HF)andaorticstenosis.AlthoughexperimentalstudiessuggestthatDNMT3ACH-drivermutationsmayenhanceinflammation,specificsignaturesofinflammatorycellsinhumansaremissing.Objective:Todefinesubsetsofimmunecellsmediatinginflammationinhumansusingsingle-cellRNA-sequencing.MethodsandResults:TranscriptomicprofilesofperipheralbloodmononuclearcellswereanalysedinN=6HFpatientsharboringDNMT3ACH-drivermutationsandN=4patientswithHFandnoDNMT3Amutationsbysingle-cellRNA-sequencing.MonocytesofHFpatientscarryingDNMT3Amutationsdemonstratedasignificantlyincreasedexpressionofinflammatorygenes